EXPLORING THE ANTIVIRAL MECHANISMS OF RAINBOW TROUT VIG-3
Shanee Herrington-Krause, Wilfrid Laurier University
SHANEE L. HERRINGTON-KRAUSE, S.J. POYNTER AND S.J. DEWITTE-ORR
Department of Biology, Wilfrid Laurier University, Waterloo ON. Canada.
Rainbow trout is the most farmed fish in Ontario and pathogen infections, including virus infections, cause catastrophic mortality rates and substantial economic losses. Despite this, there is a lack of understanding regarding fish innate immunity, specifically with regards to interferonstimulated genes (ISGs) and their antiviral effects. Viral Hemorrhagic Septicemia Virus (VHSV) induced gene (vig)-3 in rainbow trout is homologous to ISG15 in mammals. It is a small ubiquitinlike protein inducible by type I interferons (IFN), suggested to have antiviral effects within the cell. Vig-3’s antiviral activity is proposed to act both intracellularly through covalent modification of proteins, as well as extracellularly as a signaling molecule. The proposed project aims to investigate the role of vig-3 in rainbow trout antiviral innate immunity. To do this, rainbow trout gonadal cells (RTG-2) will be infected with fish viruses, and the expression of vig-3 expression will be monitored over the course of each infection. The subcellular localization of vig-3 will be observed via immunocytochemistry during viral infection, and the ability of overexpressed vig-3 to limit virus infection will be investigated. These findings will contribute to a better understanding of a poorly studied aspect of innate antiviral immunity in an economically valuable fish species.