TRANSCRIPTOMIC PROFILING OF STRONGYLOCENTROTUS PURPURATUS COELOMOCYTE POPULATIONS
Sebastian Fugmann, Chang Gung University
Fu-Yi Hsieh1, Jun Fang Cheng2, and Hsiao Han Huang2, Katherine M. Buckley3, Sebastian D
1Division of Microbiology, Graduate Institute of Biomedical Sciences, Chang Gung University, Kwei-Shan District, Tao-Yuan, Taiwan,
2Department of Biomedical Sciences, Chang Gung University, Kwei-Shan District, Tao-Yuan, Taiwan,
3Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA,
4Chang Gung Immunology Consortium, Chang Gung Memorial Hospital and Chang Gung University, Kwei-Shan District, Tao-Yuan, Taiwan.
5Department of Nephrology, Chang Gung Memorial Hospital, Tao-Yuan, Taiwan
Coelomocytes are important cellular mediators of immunity in echinoderms. In the purple sea urchin (Strongylocentrotus purpuratus) these cells can readily be divided into phagocytes, vibratile cells, red spherule cells, and colorless spherule cells based on their morphology. While some information has been reported about genes that are expressed in specific types of coelomocytes, a comprehensive analysis of the gene expression pattern in each cell type was not available. Using a density gradient coelomocytes from individual adult urchins were separated into three populations (phagocytes, a mixture of vibratile and colorless spherule cells, and red spherule cells), RNA was isolated, and next generation sequencing was employed to determine the transcriptome of each cell population. All three cell populations showed clear differences in their gene expression patterns, and the differences in transcript levels for selected genes that were found to be uniquely expressed in one cell type were confirmed by qRT-PCR using cDNA obtained from additional sea urchins. We now complemented this bulk population data with single-cell RNAseq to define coelomocyte identity and function based on their gene expression profiles. The new findings from this study will be discussed.
This work was supported by the Chang Gung Memorial Hospital (CMRPD1C0242,
CMRPD1F0421, and CORPD1F0051) and the Ministry of Science and Technology Taiwan